Name / Stock Number: Wave line DNA collection / CD4-781613

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other name: N781613

Stock Type: set

Resource Type: library

Availability: not distributed, only 60 clones from the set are available, order as individual stocks

Donors:
  • Niko Geldner

Donation Date: 11/01/2012

Date Released: 11/06/2012

Description:

The complete Wave line DNA collection: contains all marker constructs (21 different genes, available in 4 colors: 84 clones in total) and pNigel vectors. This collection is a set of distinct subcellular marker lines with spectrally distinct, fluorescent fusion constructs. The set provides an easy way to screen for distinct subcellular localization patterns and stable, non-toxic expression in transgenic plants. The constructs are under pUBQ10 promoter. The pNIGEL recombination vectors on which these constructs are based allow generation of fusion constructs by CRE-lox recombination using the pUNI ORF collection of the SSP consortium. The final marker constructs are available in four colors Blue (mCerulean), Bluegreen (mTFP1), Yellow (YFP) and Red (mCherry). The nomenclature of the constructs is, for example: Wave 131C, Wave 131T, Wave 131Y or Wave 131R, the last letter indicating the different fluorophores.

Growth Requirement: grow in LB media at 37C overnight

Marker:

Background:

ABRC Comment:

Format Shipped: individual clones shipped as frozen glycerol stocks in a 96 well plate

Express Shipping Required: true

Base / Commercial Price: $490 / $3920

Taxon: Arabidopsis thaliana 3702

Publications

DOI PubMed
10.1111/j.1365-313X.2009.03851.x 19309456

External links

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