donor stock number: T-FGC427A, A634
NASC stock number: N3987
Resource Type: seed
Availability: available
Donors:- Ed Butler
- Chromatin Functional Genomics Consortium
Donation Date: 03/20/2002
Date Released: 04/03/2002
Description:
Carries a dominant negative transgene at the HDA2 locus; generated by Agrobacterium-mediated transformation using the binary construct pFGC4273; single-insertion event (determined by Southern analysis); hygromycin-resistance co-segregating with this allele is a dominant marker; the interference portion of the transgene gives rise to a dominant negative mutation; specific reduction of target mRNA as evidenced by RT-PCR.
Growth Requirement: none
Marker: hygromycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
homozygous
dsRNA silencing
Arabidopsis thaliana 3702
Additional Information
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pFGC4273 | RNAi construct | hygromycin |
Quality Control Comments
There is no quality control data for this stock.