donor stock number: T-FGC524B
NASC stock number: N3996
Resource Type: seed
Availability: available
Donors:- Ed Butler
- Chromatin Functional Genomics Consortium
Donation Date: 03/20/2002
Date Released: 04/03/2002
Description:
Carries a dominant negative transgene at the SGA1 locus; generated by Agrobacterium-mediated transformation using the binary construct pFGC5240; single-insertion event (determined by Southern analysis); potentially confers dominant negative silencing of target gene, as well as dominant hygromycin resistance marker; specific reduction of target mRNA as evidenced by RT-PCR; gene product is homologous to yeast ASF1 (anti-silencing factor), silencing gene A group (ScASF1 like).
Growth Requirement: none
Marker: hygromycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
homozygous
dsRNA silencing
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pFGC5240 | RNAi construct | hygromycin |
Quality Control Comments
There is no quality control data for this stock.