Name / Stock Number: CS6952

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gcr1-3

N6952

seed

available

  • Sarah Assmann
  • Sona Pandey
  • 08/30/2006

    09/01/2006

    Description:

    Knockout line harboring a T-DNA insertion in intron 2 (at 506 bp) of the GCR1 gene, was found in a PCR screen of a collection of 72,960 T-DNA-inserted Arabidopsis lines (Arabidopsis Knockout Facility, University of Wisconsin, BASTA population) using a GCR1-specific primer and a T-DNA left border-specific primer; homozygous mutant line for At1g 48270 (GCR1 gene).

    none

    Ws (Wassilewskija)

    100 seeds per vial

    $13 / $104

    Germplasm

    2

    Plants exhibit hypersensitivity to abscisic acid (ABA) in assays of root growth, gene regulation, and stomatal response. Root growth of seedlings is markedly more sensitive to inhibition by ABA compared with wild-type. Plants have enhanced drought tolerance and lower rates of water loss (lower transpiration rates). Plants are hypersensitive to ABA and S1P regulation of stomatal responses: guard cells are hypersensitive to both inhibition of opening of preclosed stomata and promotion of closure of preopened stomata by ABA; plants respond to the lipid metabolite, sphingosine-1-phosphate (S1P), in an identical fashion as they do to ABA: guard cells are hypersensitive to S1P-induced inhibition of stomatal opening and S1P-induced promotion of stomatal closure compared with the wild-type.

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.020321 15155892
    Transgene Description Promoter Reporter Marker
    pSKI15 activation tag T-DNA 4x CaMV 35S BASTA

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