donor stock number: gcr1-3
NASC stock number: N6952
Resource Type: seed
Availability: available
Donors:- Sona Pandey
- Sarah Assmann
Donation Date: 08/30/2006
Date Released: 09/01/2006
Description:
Knockout line harboring a T-DNA insertion in intron 2 (at 506 bp) of the GCR1 gene, was found in a PCR screen of a collection of 72,960 T-DNA-inserted Arabidopsis lines (Arabidopsis Knockout Facility, University of Wisconsin, BASTA population) using a GCR1-specific primer and a T-DNA left border-specific primer; homozygous mutant line for At1g 48270 (GCR1 gene).
Growth Requirement: none
Marker:
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Plants exhibit hypersensitivity to abscisic acid (ABA) in assays of root growth, gene regulation, and stomatal response. Root growth of seedlings is markedly more sensitive to inhibition by ABA compared with wild-type. Plants have enhanced drought tolerance and lower rates of water loss (lower transpiration rates). Plants are hypersensitive to ABA and S1P regulation of stomatal responses: guard cells are hypersensitive to both inhibition of opening of preclosed stomata and promotion of closure of preopened stomata by ABA; plants respond to the lipid metabolite, sphingosine-1-phosphate (S1P), in an identical fashion as they do to ABA: guard cells are hypersensitive to S1P-induced inhibition of stomatal opening and S1P-induced promotion of stomatal closure compared with the wild-type.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.020321 | 15155892 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSKI15 | activation tag T-DNA | 4x CaMV 35S | BASTA |
Quality Control Comments
There is no quality control data for this stock.