Name / Stock Number: SAIL_221_H01 / CS810254

NASC stock number: N810254

Resource Type: seed

Availability: available

Donors:
  • Syngenta, USA

Donation Date: 03/01/2004

Date Released: 06/01/2004

Description:

T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1105/tpc.004630 12468722
Transgene Description Promoter Reporter Marker
pCSA110 T-DNA LAT52 GUS BASTA

Quality Control Comments

Comment Commented by Date
new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here