Name / Stock Number: WiscDsLox318B05 / CS851020

NASC stock number: N851020

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan
  • Michael Sussman
  • Rick Amasino

Donation Date: 09/21/2004

Date Released: 12/01/2004

Description:

Sequence-indexed T-DNA insertion line; the T-DNA includes a Ds transposon and a LoxP recombination site. A Basta resistance marker is used to select for the T-DNA and for the Ds transposon following any transposition. However, be aware that lines from this set might not be able to launch their Ds element. Be sure to test any line for the ability to transpose before using it in experiments. Hygromycin resistance can be used for selection of lines where the Ds element has been excised by transposition. WiscDsLox lines do not contain Ac transposase, and the lines should be crossed to a line expressing Ac transposase in order to mobilize the Ds transposon. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $13 / $104

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
pDs-Lox T-DNA includes a Ds transposon and a LoxP recombination site CaMV35S, MAS LUC hygromycin; BASTA

Quality Control Comments

Comment Commented by Date
new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here