NASC stock number: N870323
Resource Type: seed
Availability: available, note that a confirmed line has been isolated from this stock
Donors:- Syngenta, USA
Donation Date: 04/06/2006
Date Released: 06/21/2006
Description:
T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Visible differences between mutant plants and their wild type siblings were modest. The growth rate of mutant plants was 20% lower, and they showed a flowering delay of about 1 week. There was no difference in leaf color or form.
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1105/tpc.004630 | 12468722 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pCSA110 | T-DNA | LAT52 | GUS | BASTA |
Quality Control Comments
There is no quality control data for this stock.