Name / Stock Number: WiscDsLoxHs091_03A / CS908657

donor stock number: DsLoxHs091_03A

NASC stock number: N908657

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 04/16/2009

Date Released: 05/14/2009

Description:

Sequence-indexed T-DNA insertion line, generated by Agrobacterium-mediated transformation of Columbia (Col) plants with the T-DNA vector pDsLoxHs. Primary transformants were selected on Kanamycin plates. The pDsLoxHs vector contains a launchpad for a Ds transposon, as well as sites for Cre/ Lox recombination. A gene encoding the Ac transposase is also present on the T-DNA, under the control of a heat-shock inducible promoter. Two LoxP recombination sites are present in pDsLoxHs: one LoxP site is within the Ds portion of the vector and the other outside of the Ds element. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

Transgene Description Promoter Reporter Marker
pDsLoxHs T-DNA with Ds launch pad heat-shock inducible kanamycin

Quality Control Comments

Comment Commented by Date
new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here