donor stock number: PtGFP_kn
NASC stock number: N9561
Resource Type: seed
Availability: available
Donors:- Christoph Plieth
Donation Date: 09/17/2008
Date Released: 12/10/2009
Description:
Transgenic line; constitutively expresses green fluorescent protein (PtGFP) from the seapen Ptilosarcus gurneyi under the control of a single 35S CaMV promoter; PtGFP is a pH-reporter protein when used ratiometrically in the double excitation mode having a broad pH-responsiveness and a good acid stability; can be used to monitor cytoplasmic pH in a wide range of applications
Growth Requirement: kanamycin resistant
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
cytoplasm fluoresces green when exited with blue light; no other differences in appearance, growth, development or reproduction when compared to Col-0 wild type
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1186/1746-4811-2-7 | 16600023 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| 35S:Pt-GFP | constitutively expresses green fluorescent protein (PtGFP) from the seapen Ptilosarcus gurneyi under the control of a single 35S CaMV promoter | CaMV 35S | GFP from seapen (Ptilosarcus gurneyi) | kanamycin |
Quality Control Comments
There is no quality control data for this stock.