Name / Stock Number: CS9750

NASC stock number: N9750

donor stock number: HET:80c -b5

Resource Type: seed

Availability: available

Donors:
  • Ian Moore

Donation Date: 12/18/2009

Date Released: 10/22/2010

Description:

Enhancer trap line generated with the vector pHET-LhG4 for use in the pOp/LhG4 system. The pOp/LhG4 system is used for spatial and temporal control of transgene expression in plants. This is based on a chimeric transcription factor, LhG4, comprising a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein. This molecule activates transcription from the pOp promoter which is otherwise physiologically silent in transgenic plants. Some lines still segregate for the enhancer trap and can be selected on kanamycin.

Growth Requirement: kanamycin resistant

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1111/j.1365-313X.2005.02486.x 16115072
Transgene Description Promoter Reporter Marker
pHET-LhG4 enhancer trap T-DNA containing a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein pOp/LhG4 GUS kanamycin

Quality Control Comments

There is no quality control data for this stock.