Name / Stock Number: CS9767

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HET:82f -t1

N9767

seed

available

  • Ian Moore
  • 12/18/2009

    10/22/2010

    Description:

    Enhancer trap line generated with the vector pHET-LhG4 for use in the pOp/LhG4 system. The pOp/LhG4 system is used for spatial and temporal control of transgene expression in plants. This is based on a chimeric transcription factor, LhG4, comprising a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein. This molecule activates transcription from the pOp promoter which is otherwise physiologically silent in transgenic plants. Some lines still segregate for the enhancer trap and can be selected on kanamycin.

    kanamycin resistant

    Col (Columbia)

    100 seeds per vial

    $13 / $104

    Germplasm

    2

    GUS expression in shoot apex and petioles

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1111/j.1365-313X.2005.02486.x 16115072
    Transgene Description Promoter Reporter Marker
    pHET-LhG4 enhancer trap T-DNA containing a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein pOp/LhG4 GUS kanamycin

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