NASC stock number: N9769
donor stock number: HET:85c t1.5
Resource Type: seed
Availability: available
Donors:- Ian Moore
Donation Date: 12/18/2009
Date Released: 10/22/2010
Description:
Enhancer trap line generated with the vector pHET-LhG4 for use in the pOp/LhG4 system. The pOp/LhG4 system is used for spatial and temporal control of transgene expression in plants. This is based on a chimeric transcription factor, LhG4, comprising a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein. This molecule activates transcription from the pOp promoter which is otherwise physiologically silent in transgenic plants. Some lines still segregate for the enhancer trap and can be selected on kanamycin.
Growth Requirement: kanamycin resistant
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1111/j.1365-313X.2005.02486.x | 16115072 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pHET-LhG4 | enhancer trap T-DNA containing a high-affinity DNA-binding mutant of the E. coli lac repressor fused to a transcription activation domain from the yeast Gal4 protein | pOp/LhG4 | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.