Name / Stock Number: CS99347

NASC stock number: N99347

donor stock number: TEC-SCHP-5-2

Stock Type: pool

Resource Type: seed

Availability: available

Donors:
  • Julian Schroeder
  • Felix Hauser

Donation Date: 10/26/2017

Date Released: 06/11/2019

Description:

This subpool (CS99347) consists of 90 independent transformants expressing artificial microRNAs (amiRNAs) designed to target homologous, possibly functionally redundant genes in forward genetic screens; represents a subpool of TEC amiRNA pool CS99431. Note that the larger pool CS99431 is recommended for forward genetic screening of many lines. Generated by transforming Col-0 plants with a combination of 20bp and 21bp amiRNA libraries (CD4-62 and CD4-72, respectively) in p35S::amiRNA3 OCS (pPHANTOM vector) targeting transcripts encoding metabolic and other enzymes catalyzing transfer reactions (EC: 2) (TEC); protein family member functional classes: CHO metabolism, glycolysis, gluconeogenesis, TCA cycle, amino acid metabolism, secondary metabolism, hormone metabolism, transferases, lipid metabolism (Mapman); transferase activity (GO:0016740). Basta resistant.

Growth Requirement: Select on BASTA plates or with Finale for selection on soil

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 1250 seeds per vial

Base / Commercial Price: $50 / $400

Germplasm

2

Mixture of phenotypes depending on unknown amiRNA expressed.

Arabidopsis thaliana 3702

Additional Information

Quality Control Comments

There is no quality control data for this stock.