NASC stock number: N664857
Resource Type: seed
Availability: available
Donors:- Joseph Ecker
- SALK Confirmed T-DNA Project
Donation Date: 07/20/2007
Date Released: 07/25/2007
Description:
Sequence-indexed T-DNA insertion line; confirmed line isolated from original SALK line; presence of the insertion was analyzed by PCR.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment: NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating.; Please cite the Alonso et al. reference linked to this stock and acknowledge ABRC for distributing the seeds in all presentations and publications utilizing this material.
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1126/science.1086391 | 12893945 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
pROK2 | T-DNA | kanamycin |
Quality Control Comments
Comment | Commented by | Date | |
---|---|---|---|
researcher reported: We obtained this line and found that there is a segregating mutation. The phenotype is similar to axr1 (slow growth, reduced apical dominance). We've used primer sets to try to validate the insert but in no case do either class of plants (WT and the axr1-like) give rise to a PCR product with a gene specific/T-DNA primer pair. | ABRC | 11/15/2012 | To view detailed data, click here |