Name / Stock Number: SALK_120804C

NASC stock number: N664857

Resource Type: seed

Availability: available

Donors:
  • Joseph Ecker
  • SALK Confirmed T-DNA Project

Donation Date: 07/20/2007

Date Released: 07/25/2007

Description:

Sequence-indexed T-DNA insertion line; confirmed line isolated from original SALK line; presence of the insertion was analyzed by PCR.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment: NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating.; Please cite the Alonso et al. reference linked to this stock and acknowledge ABRC for distributing the seeds in all presentations and publications utilizing this material.

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1126/science.1086391 12893945
Transgene Description Promoter Reporter Marker
pROK2 T-DNA kanamycin

Quality Control Comments

Comment Commented by Date
researcher reported: We obtained this line and found that there is a segregating mutation. The phenotype is similar to axr1 (slow growth, reduced apical dominance). We've used primer sets to try to validate the insert but in no case do either class of plants (WT and the axr1-like) give rise to a PCR product with a gene specific/T-DNA primer pair. ABRC 11/15/2012 To view detailed data, click here