The methods used by the ABRC for handling Arabidopsis thaliana (Arabidopsis) plants and seeds are outlined in the PDF below. These procedures are designed to generate healthy plants that give the maximum set of pure seeds and to preserve these in the safest and most convenient manner. The plant and seed management protocols are given in the chronological order in which they would normally be utilized. These methods are used by the ABRC for handling plants and seeds. Many other approaches may be as good, especially in specific experimental situations. If you have any questions concerning the procedures, feel free to contact us.
Rivero L., Scholl R., Holomuzki N., Crist D., Grotewold E., Brkljacic J. (2014) Handling Arabidopsis Plants: Growth, Preservation of Seeds, Transformation, and Genetic Crosses. In: Sanchez-Serrano J., Salinas J. (eds) Arabidopsis Protocols. Methods in Molecular Biology (Methods and Protocols), vol 1062. Humana Press, Totowa, NJ
The video below shows basic planting and quality control procedures at ABRC
[pdf] - Some links on this page are to .pdf files. If you need these files in a more accessible format, please contact us. PDF files require the use of Adobe Acrobat Reader software to open them. If you do not have Reader, you may use the following link to Adobe to download it for free at: Adobe Acrobat Reader.