NASC stock number: N2110814
donor stock number: cle41 cle42 cle43 cle44
other name: tdif
Resource Type: seed
Availability: available
Donors:- Peter Etchells
Donation Date: 09/13/2021
Date Released: 01/12/2022
Description:
cle41 cle42 cle43 cle44 quadruple mutant (tdif) generated using CRISPR. For each of the four targeted CLE genes, a 20-bp guide RNA (gRNA) target site was selected. A gRNA gene array was synthesized as a group of four AtU6:gRNA tandem constructs, which was subsequently cloned into the pCUT4 binary vector. Col-0 plants were transformed with the CRISPR binary construct via the floral dip method. Hygromycin resistant.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
anthacyanin build up in leaves and cotyledons
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.19.00562 | 31806676 |
Quality Control Comments
There is no quality control data for this stock.