ABRC
Arabidopsis Biological Resource Center

Description:

transgenic line carrying reporter construct for A. thaliana mitotic cyclins; translational fusion of mitotic cyclin with the GUS reporter gene; the vector employed was pMDC163 (CD3-755); the construct consists of an entire cyclin genic sequence including introns and ~2 kb upstream promoter region fused to the GUS cDNA at the position of the termination codon; 3 prime UTR is derived from the nopaline synthase gene; approximately 20 independent T1 transgenic were prescreened by histochemical GUS staining of flowers, and progeny of three plants with the most representative staining pattern were used for in depth expression analyses in T2 and T3; seeds of T4 generation of one representative line were donated to the stock centers

Growth Requirement: none

Marker: hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Publications

Transgenes