donor stock number: SALK_129163
NASC stock number: N6934
Resource Type: seed
Availability: available
Donors:- Georg Jander
- Vijay Joshi
Donation Date: 11/14/2006
Date Released: 01/16/2007
Description:
confirmed line isolated from original SALK line; heterozygous for the insertion; kanamycin resistant (25ug/ml).
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
25% of seeds in developing silique of heterozygous plants are albino and lethal; mature mutant and wild type seeds look identical; but 25% of the developing seedlings are albino, fail to mature beyond the cotyledon stage and do not accumulate any chlorophyll pigments. Mutant growth not rescued with exogenous addition of metabolically related amino acids. Significant alteration in the amino acid profiles of most plant tissue types, increased seed Threonine (Thr) content; reduced Glicyn (a product of Thr aldolase reaction) content in all plant tissues; increased branched-chain amino acids (Ile, Leu and Val) in vegetative tissues, except in stems, where there is a decrease. Reduced levels of organic acids and sugars, citrate and isocitrate levels in seeds and flowers are significantly lower than in wild type; levels of most sugars are significantly decreased in flowers and siliques.
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.106.044958 | 17172352 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.