ABRC
donor stock number: dKO
NASC stock number: N860079
Resource Type: seed
Availability: available
Donors:- Eva Collakova
- Yair Shachar-Hill
- Andrew Hanson
Donation Date: 07/07/2008
Date Released: 08/11/2008
Description:
Double mutant (dKO = double knockout) generated by crossing SALK_062946 (CS860076) and Garlic_169F06 (CS860077) homozygous lines; double homozygous plants were confirmed by PCR.
Growth Requirement: plants have to be grown at high CO2 atmosphere (~4000 ppm) to obtain viable seeds.
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Phenotype: Severe photorespiratory phenotypes: plants are small, pale, delayed in flowering and infertile; plants show a 70-fold increase in glycine levels and accumulate higher levels of 5- and 10-formyl tetrahydrofolate (THF) than the wild type; levels of amino acids and sugars are altered; leaf folate levels and composition are also altered; embryo development arrests between heart and early bent cotyledon stages; mature seeds are shriveled, carrying colorless embryos, accumulate low amounts of lipids, and fail to germinate; conditionally lethal, rescued by growth under non-photorespiratory (high CO2) conditions; culturing siliques in the presence of sucrose restores normal embryo development and seed viability.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1105/tpc.108.058701 | 18628352 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pCSA110 | T-DNA | LAT52 | GUS | BASTA |
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
There is no quality control data for this stock.