Arabidopsis Biological Resource Center

Description:

Line isolated by PCR screening the multidimensional DNA pools of T-DNA insertion mutant population generated in the Col-0 background at the Salk Institute. The T-DNA left border primer and degenerate primer 244 was used for PCR. Mutant line was identified by analyzing PCR products by DNA-blot analyses using gene-specific cDNA probe for the gene of interest, and the location of the T-DNA insertion was determined by DNA sequence analysis of the PCR products. Homozygous mutant plants were identified by PCR using allele-specific primers. To detect any remaining transcript produced, RT-PCR was performed using primers that span the T-DNA insertion and are specific for sequences upstream of the insertion. Backcrossed once to wild type.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120