Name / Stock Number: CS16352

View Order History

rga-28

N16352

seed

available

  • Tai-ping Sun
  • 08/21/2008

    09/04/2008

    Description:

    Line isolated by PCR screening the multidimensional DNA pools of T-DNA insertion mutant population generated in the Col-0 background at the Salk Institute. The T-DNA left border primer and degenerate primer 244 was used for PCR. Mutant line was identified by analyzing PCR products by DNA-blot analyses using gene-specific cDNA probe for the gene of interest, and the location of the T-DNA insertion was determined by DNA sequence analysis of the PCR products. Homozygous mutant plants were identified by PCR using allele-specific primers. To detect any remaining transcript produced, RT-PCR was performed using primers that span the T-DNA insertion and are specific for sequences upstream of the insertion. Backcrossed once to wild type.

    none

    Col (Columbia)

    100 seeds per vial

    $13 / $104

    Germplasm

    2

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1104/pp.104.039578 15173565
    Transgene Description Promoter Reporter Marker
    pROK2 T-DNA kanamycin

    Quality Control Comments

    There is no quality control data for this stock. Add your quality control comment here