Name / Stock Number: WiscDsLoxHs012_07A / CS901105

donor stock number: DsLoxHs012_07A

NASC stock number: N901105

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 10/15/2008

Date Released: 11/07/2008

Description:

Sequence-indexed T-DNA insertion line, generated by Agrobacterium-mediated transformation of Columbia (Col) plants with the T-DNA vector pDsLoxHs. Primary transformants were selected on Kanamycin plates. The pDsLoxHs vector contains a launchpad for a Ds transposon, as well as sites for Cre/ Lox recombination. A gene encoding the Ac transposase is also present on the T-DNA, under the control of a heat-shock inducible promoter. Two LoxP recombination sites are present in pDsLoxHs: one LoxP site is within the Ds portion of the vector and the other outside of the Ds element. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT3G56190
  • ASNAP
  • ALPHA-SNAP2
  • alpha-soluble NSF attachment protein 2
  • polymorphism SIGnAL TAIR ARAPORT
    AT5G39550
  • ORTHRUS 1
  • VARIANT IN METHYLATION 3
  • ORTH1
  • VIM3
  • polymorphism SIGnAL TAIR ARAPORT
    AT5G39540 polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    Wiscseq_DsLoxHs012_07A.1 T-DNA insertion segregating
  • AT5G39540
  • AT5G39550
  • SIGnAL
    WiscDsLoxHs012_07A T-DNA insertion segregating
  • AT3G56190
  • SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    Transgene Description Promoter Reporter Marker
    pDsLoxHs T-DNA with Ds launch pad heat-shock inducible kanamycin

    Quality Control Comments

    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here