Name / Stock Number: WiscDsLoxHs181_06H / CS917328

donor stock number: DsLoxHs181_06H

NASC stock number: N917328

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan

Donation Date: 08/12/2009

Date Released: 09/05/2009

Description:

Sequence-indexed T-DNA insertion line, generated by Agrobacterium-mediated transformation of Columbia (Col) plants with the T-DNA vector pDsLoxHs. Primary transformants were selected on Kanamycin plates. The pDsLoxHs vector contains a launchpad for a Ds transposon, as well as sites for Cre/ Lox recombination. A gene encoding the Ac transposase is also present on the T-DNA, under the control of a heat-shock inducible promoter. Two LoxP recombination sites are present in pDsLoxHs: one LoxP site is within the Ds portion of the vector and the other outside of the Ds element. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT3G61820 polymorphism SIGnAL TAIR ARAPORT

Germplasm

2

Polymorphisms

Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
Wiscseq_DsLoxHs181_06H.0 T-DNA insertion segregating
  • AT3G61820
  • SIGnAL
    WiscDsLoxHs181_06H T-DNA insertion segregating
  • AT3G61820
  • SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    Transgene Description Promoter Reporter Marker
    pDsLoxHs T-DNA with Ds launch pad heat-shock inducible kanamycin

    Quality Control Comments

    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here