donor stock number: DsLoxHs181_06H
NASC stock number: N917328
Resource Type: seed
Availability: available
Donors:- Patrick Krysan
Donation Date: 08/12/2009
Date Released: 09/05/2009
Description:
Sequence-indexed T-DNA insertion line, generated by Agrobacterium-mediated transformation of Columbia (Col) plants with the T-DNA vector pDsLoxHs. Primary transformants were selected on Kanamycin plates. The pDsLoxHs vector contains a launchpad for a Ds transposon, as well as sites for Cre/ Lox recombination. A gene encoding the Ac transposase is also present on the T-DNA, under the control of a heat-shock inducible promoter. Two LoxP recombination sites are present in pDsLoxHs: one LoxP site is within the Ds portion of the vector and the other outside of the Ds element. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Polymorphisms
| Polymorphism | Mutagen | Genotype | Inheritance | Gene | Evidence attribution |
|---|---|---|---|---|---|
| Wiscseq_DsLoxHs181_06H.0 | T-DNA insertion | segregating | SIGnAL | ||
| WiscDsLoxHs181_06H | T-DNA insertion | segregating | SIGnAL |
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pDsLoxHs | T-DNA with Ds launch pad | heat-shock inducible | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute | ABRC | 03/10/2015 | To view detailed data, click here |