NASC stock number: N66064
donor stock number: pSHR:HF-RPL18 (10-1-2)
Resource Type: seed
Availability: available
Donors:- Julia Bailey-Serres
Donation Date: 08/11/2010
Date Released: 09/28/2010
Description:
Transgenic line; A construct containing the SHR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene was cloned into the binary vector pGATA-S119 that carries Kanamycin resistance. The binary vector was electroporated into A.tumefaciens strain LBA4404, which was used for plant transformation. This line is homozygous for the insertion and the insertion site is known. Users are strongly encouraged to confirm the genotype by use of genomic PCR. A primer list and protocols for the use of these lines will be posted on the donor's website (see URL link).
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
homozygous
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1073/pnas.0906131106 | 19843695 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pSHR:HF-RPL18 | SHR promoter, TMV omega leader sequence, His6-FLAG tag, coding region of RPL18B and 3' UTR of the OSC gene | SHR |
Quality Control Comments
There is no quality control data for this stock.