NASC stock number: N66068
donor stock number: mSPL13-2D
Resource Type: seed
Availability: available
Donors:- Hiro Nonogaki
Donation Date: 08/18/2010
Date Released: 10/21/2010
Description:
The SPL13 gene including the 1.3 kb upstream regulatory region was amplified from genomic DNA of Arabidopsis thaliana ecotype Col-0. Mutations at the miR156 target site were generated by site overlap extension PCR mutagenesis (Ho et al., 1989). PCR products were cloned into pCAMBIA1301 and Col-0 plants were transformed by floral dip method. Transgenic plants carry Hygromycin resistance. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Slightly delayed leaf development.
Arabidopsis thaliana 3702
Additional Information
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| mSPL13 | mutated SPL13 gene including the 1.3 kb upstream regulatory region | hygromycin |
Quality Control Comments
There is no quality control data for this stock.