NASC stock number: N9962
donor stock number: PRR9:LUC+
Resource Type: seed
Availability: available
Donors:- Andrew Millar
Donation Date: 06/27/2011
Date Released: 02/22/2012
Description:
Transgenic line; fusion of PRR9 upstream region to the LUC+ firefly luciferase reporter gene; a 1330 bp PRR9 promoter fragment was obtained by PCR performed on genomic DNA of wild type plants and cloned in the pPCV-LUC+ binary vector; the amplified fragment contained the entire 5'-untranslated region, but not the ATG; transformants were selected with 15 æg mL1 of hygromycin
Growth Requirement: hygromycin resistant (15 ug/ml)
Marker: hygromycin
Background: Ws (Wassilewskija)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
DOI | PubMed |
---|---|
10.1038/msb.2010.81 | 21045818 |
Transgene | Description | Promoter | Reporter | Marker |
---|---|---|---|---|
PRR9:LUC | 1330 bp PRR9 promoter fragment fused to the LUC+ firefly luciferase reporter gene | PRR9 | LUC | hygromycin |
Quality Control Comments
There is no quality control data for this stock.