ABRC
NASC stock number: N67770
other name: rhd2-1
donor stock number: rhd2 WER::GFP
Resource Type: seed
Availability: available
Donors:- John Schiefelbein
Donation Date: 03/23/2012
Date Released: 04/24/2012
Description:
The rhd2 (CS2259) mutant line was crossed with a specific WER::GFP (CS66493) transgenic line, which contains a single transgene on chromosome five. Kanamycin resistant. Genotype for transgene is unknown.
Growth Requirement: Kanamycin resistant.
Marker: kanamycin
Background:
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Short and stubby root hairs. GFP is expressed in all stages of differentiating root epidermal cells, in both hair (H) and non-hair (N) cell types, although preferentially in the N cells.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1371/journal.pgen.1002446 | 22253603 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| WER::GFP | 2.5 kb 5' fragment and a 1.1 kb 3' fragment from the WER gene fused to mGFP5 | WER | GFP | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Researcher reported low germination | ABRC | 04/11/2018 | To view detailed data, click here |
| Germination was found to be 97%, which is within the acceptable range for germination percentage (81-100%) set by ABRC. | ABRC | 04/24/2018 | To view detailed data, click here |