Name / Stock Number: CS98345

NASC stock number: N98345

donor stock number: set

Stock Type: set

Resource Type: set of seeds

Availability: available

  • Maarten Koornneef

Donation Date: 03/07/2013

Date Released: 04/05/2013


Set of 530 Arabidopsis multiparent recombinant inbred lines (AMPRIL). Eight founder accessions (Col, Kyo-1, Cvi, Sha, Eri-1, An-1, Ler, and C24) were crossed to produce six recombinant inbred line (RIL) sub populations. Founders were crossed pairwise to produce four F1 hybrids (A, Col x Kyo-1; B, Cvi x Sha; C, Eri-1 x An-1; and D, Ler x C24). These F1s were crossed according to a diallel scheme (AB, AC, AD, BC, BD, and CD) In all crosses, the first parent is the female. The resulting offspring was then selfed for three generations by single-seed descent. The F4 generation was genotyped with SNP and microsatellite markers. Data for flowering time and leaf morphology traits were determined in the F5 generation. Distribution seeds are the progeny of a F5 single-seed plant, where residual heterozygosity was present in the parent line (see genetic marker data). This implies that each F4 plant was still heterozygous for an average of 40 loci (in agreement with what was expected theoretically). This residual heterozygosity allows selection of heterogenous families for the QTL of interest. Some of the progeny might have become homozygous due to selfing.

Growth Requirement: none



ABRC Comment: The parental lines are included with this stock

Format Shipped: 100 seeds per vial

Base / Commercial Price: $2595 / $20760

Arabidopsis thaliana 3702

Additional Information

Quality Control Comments

There is no quality control data for this stock.