Name / Stock Number: CS67936

NASC stock number: N67936

donor stock number: fly1-1

Resource Type: seed

Availability: available

Donors:
  • George Haughn

Donation Date: 05/03/2013

Date Released: 08/30/2013

Description:

Isolated from a population of EMS-mutagenized Col-2 plants in a screen for mutants exhibiting seed mucilage defects. Backcrossed 4 times to Col-2. Sequencing of At4g28370 in the fly1-1 background revealed a G-to-A transition, consistent with EMS mutation, at 3903 bp that changes the amino acid Trp (residue 460) to a stop codon. This early stop codon is predicted to truncate the peptide encoded by the FLY1 gene, resulting in the loss of the conserved RING domain.

Growth Requirement: none

Marker:

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

Ploidy: 2

Reduced seed mucilage extrusion, increased mucilage adherence, detached outer tangential primary cell walls from the columellae of seed coat epidermal cells (forming discs around the mucilage halo).

Taxon: Arabidopsis thaliana 3702

Publications

DOI PubMed
10.1105/tpc.112.107888 23482858

Quality Control Comments

There is no quality control data for this stock.