Name / Stock Number: CS68211

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NASC stock number: N68211

other name: p35S SBP_R1_amiRNA

donor stock number: 72-1T2

Resource Type: seed

Availability: not distributed, no seeds at ABRC

Donors:
  • Felix Hauser
  • Julian Schroeder

Donation Date: 10/23/2013

Date Released: 06/02/2014

Description:

AmiRNA line. Original donation was T2 generation of the TPK amiRNA library. AmiRNA sequence and primers were designed using the WMD Design Tool online. AmiRNA PCR product was cloned into pENTR D TOPO, then transferred to vector pRS1102 using the LR clonase method. pSOUP (stock number CD3-1124) used as a helper plasmid for transformation. Using the floral dip method, pRAB18:GFP plants (CS68523) were transformed. Heterozygous. Basta resistant. Predicted targeted loci: AT1G27360; AT1G27370; AT1G53160; AT1G69170; AT2G33810; AT2G42200; AT3G57920; AT5G43270; AT5G50570; AT5G50670; amiRNA sequence: TGACAGAAGAGAGAGAGCACC; Listed targeted loci are predictions, and are not experimentally verified.

Growth Requirement: Basta resistant

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT1G27370
  • SPL10
  • squamosa promoter binding protein-like 10
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G53160
  • FTM6
  • SPL4
  • FLORAL TRANSITION AT THE MERISTEM6
  • squamosa promoter binding protein-like 4
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G69170
  • AtSPL6
  • SPL6
  • SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-domain transcription factor 6
    		•  transgene SIGnAL TAIR ARAPORT
    AT2G33810
  • SPL3
  • squamosa promoter binding protein-like 3
    		•  transgene SIGnAL TAIR ARAPORT
    AT2G42200
  • AtSPL9
  • SPL9
  • squamosa promoter binding protein-like 9
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G57920
  • SPL15
  • squamosa promoter binding protein-like 15
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G43270
  • squamosa promoter binding protein-like 2
  • SPL2
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G50570
  • SQUAMOSA PROMOTER-BINDING PROTEIN LIKE 13
  • SQUAMOSA PROMOTER-BINDING PROTEIN LIKE 13A
  • SPL13
  • SPL13A
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G50670
  • SQUAMOSA PROMOTER-BINDING PROTEIN LIKE 13
  • SQUAMOSA PROMOTER-BINDING PROTEIN LIKE 13B
  • SPL13
  • SPL13B
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G66400
  • ARABIDOPSIS THALIANA DROUGHT-INDUCED 8
  • RESPONSIVE TO ABA 18
  • ATDI8
  • RAB18
    		•  transgene SIGnAL TAIR ARAPORT

    Germplasm

    2

    Similar phenotype to miRNA 156b overexpression lines. Moderate delay in flowering under long days, initiate rosette leaves faster than wild-type under short days, severe decrease of apical dominance, and increased leaf number ((PMID: 15809034).

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.113.112805 23956262
    Transgene Description Promoter Reporter Marker
    pRAB18:GFP RAB18 GFP kanamycin
    pFH0072 RNAi construct BASTA

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