Name / Stock Number: CS68212

NASC stock number: N68212

other name: p35S SBP_R1_amiRNA

donor stock number: 72-2T2

Resource Type: seed

Availability: not distributed, no seeds at ABRC

Donors:
  • Felix Hauser
  • Julian Schroeder

Donation Date: 10/23/2013

Date Released: 06/02/2014

Description:

AmiRNA line. Original donation was T2 generation of the TPK amiRNA library. AmiRNA sequence and primers were designed using the WMD Design Tool online. AmiRNA PCR product was cloned into pENTR D TOPO, then transferred to vector pRS1102 using the LR clonase method. pSOUP (stock number CD3-1124) used as a helper plasmid for transformation. Using the floral dip method, pRAB18:GFP plants (CS68523) were transformed. Heterozygous. Basta resistant. Predicted targeted loci: AT1G27360; AT1G27370; AT1G53160; AT1G69170; AT2G33810; AT2G42200; AT3G57920; AT5G43270; AT5G50570; AT5G50670; amiRNA sequence: TGACAGAAGAGAGAGAGCACC; Listed targeted loci are predictions, and are not experimentally verified.

Growth Requirement: Basta resistant

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

2

Similar phenotype to miRNA 156b overexpression lines. Moderate delay in flowering under long days, initiate rosette leaves faster than wild-type under short days, severe decrease of apical dominance, and increased leaf number ((PMID: 15809034).

Arabidopsis thaliana 3702

Additional Information

DOI PubMed
10.1105/tpc.113.112805 23956262
Transgene Description Promoter Reporter Marker
pRAB18:GFP RAB18 GFP kanamycin
pFH0072 RNAi construct BASTA

Quality Control Comments

There is no quality control data for this stock.