ABRC
NASC stock number: N68214
other name: p35S zfHD R1 amiRNA
donor stock number: 79-1T2
Resource Type: seed
Availability: available
Donors:- Felix Hauser
- Julian Schroeder
Donation Date: 10/23/2013
Date Released: 06/02/2014
Description:
AmiRNA line. Original donation was T2 generation of the TPK amiRNA library. AmiRNA sequence and primers were designed using the WMD Design Tool online. AmiRNA PCR product was cloned into pENTR D TOPO, then transferred to vector pRS1102 using the LR clonase method. pSOUP (stock number CD3-1124) used as a helper plasmid for transformation. Using the floral dip method, pRAB18:GFP plants (CS68523) were transformed. Predicted targeted loci: AT1G69600; AT1G75240; AT3G28920; AT4G24660; AT5G15210; AT5G39760; AT5G42780; AT5G60480; AT5G65410; amiRNA sequence: TGGTGGAAGTTACGGTGGCAG. Heterozygous. Basta resistant. Listed targeted loci are predictions, and are not experimentally verified.
Growth Requirement: Basta resistant
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Narrower and elongated leaf planes. Reduced apical dominance and shorter siliques. The glossy leaf surface is not due to a lack of trichomes.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1105/tpc.113.112805 | 23956262 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pRAB18:GFP | RAB18 | GFP | kanamycin | |
| pFH0079 | RNAi construct | BASTA |
Quality Control Comments
There is no quality control data for this stock.