Name / Stock Number: CS68276

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NASC stock number: N68276

other name: p35S RWP-RK Y1 amiRNA

donor stock number: 123-4T2

Resource Type: seed

Availability: available

Donors:
  • Felix Hauser
  • Julian Schroeder

Donation Date: 10/23/2013

Date Released: 06/02/2014

Description:

AmiRNA line. Original donation was T2 generation of the TPK amiRNA library. AmiRNA sequence and primers were designed using the WMD Design Tool online. AmiRNA PCR product was cloned into pENTR D TOPO, then transferred to vector pRS1102 using the LR clonase method. pSOUP (stock number CD3-1124) used as a helper plasmid for transformation. Using the floral dip method, pRAB18:GFP plants (CS68523) were transformed. Heterozygous. Basta resistant. Listed targeted loci are predictions, and are not experimentally verified. Predicted targeted loci: AT1G20640; AT1G64530; AT1G76350; AT2G17150; AT4G24020; amiRNA sequence: TACATTAGGAGTCCACTCAGG. Heterozygous. Basta resistant. Listed targeted loci are predictions, and are not experimentally verified.

Growth Requirement: Basta resistant

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT1G20640
  • NLP4
  • NIN-like protein 4
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G64530
  • NLP6
  • NIN-like protein 6
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G76350
  • NLP5
  • NIN-like protein 5
    		•  transgene SIGnAL TAIR ARAPORT
    AT2G17150
  • NLP1
  • NIN-like protein 1
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G24020
  • NLP7
  • NIN like protein 7
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G66400
  • ARABIDOPSIS THALIANA DROUGHT-INDUCED 8
  • RESPONSIVE TO ABA 18
  • ATDI8
  • RAB18
    		•  transgene SIGnAL TAIR ARAPORT

    Germplasm

    2

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.113.112805 23956262
    Transgene Description Promoter Reporter Marker
    pRAB18:GFP RAB18 GFP kanamycin
    pFH0123 RNAi construct BASTA

    Quality Control Comments

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