Name / Stock Number: CS68509

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NASC stock number: N68509

other name: p35S NAC_m6

donor stock number: 289-1T2

Resource Type: seed

Availability: available

Donors:
  • Felix Hauser
  • Julian Schroeder

Donation Date: 10/23/2013

Date Released: 06/02/2014

Description:

AmiRNA line. Original donation was T2 generation of the TPK amiRNA library. AmiRNA sequence and primers were designed using the WMD Design Tool online. AmiRNA PCR product was cloned into pENTR D TOPO, then transferred to vector pRS1102 using the LR clonase method. pSOUP (stock number CD3-1124) used as a helper plasmid for transformation. Using the floral dip method, pRAB18:GFP plants (CS68523) were transformed. Heterozygous. Basta resistant. Listed targeted loci are predictions, and are not experimentally verified. Predicted targeted loci: AT1G02230; AT1G26870; AT1G32510; AT1G32770; AT1G32870; AT1G33060; AT1G34190; AT1G52880; AT1G52890; AT1G54330; AT1G61110; AT1G69490; AT1G76420; AT2G18060; AT2G24430; AT3G04060; AT3G04070; AT3G10480; AT3G10490; AT3G10500; AT3G12977; AT3G15170; AT3G15500; AT3G15510; AT3G17730; AT3G29035; AT3G49530; AT4G01520; AT4G01540; AT4G01550; AT4G10350; AT4G27410; AT4G35580; AT5G04410; AT5G07680; AT5G08790; AT5G17260; AT5G18270; AT5G24590; AT5G39610; AT5G46590; AT5G53950; AT5G63790; amiRNA sequence: TGATACTCATGCATGATCCAC. Heterozygous. Basta resistant. Listed targeted loci are predictions, and are not experimentally verified.

Growth Requirement: Basta resistant

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT5G66400
  • ARABIDOPSIS THALIANA DROUGHT-INDUCED 8
  • RESPONSIVE TO ABA 18
  • ATDI8
  • RAB18
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G02230
  • NAC domain containing protein 4
  • ANAC004
  • NAC004
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G26870
  • ANAC009
  • FEZ
  • Arabidopsis NAC domain containing protein 9
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G32510
  • NAC domain containing protein 11
  • ANAC011
  • NAC011
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G32770
  • AtSND1
  • NAC domain containing protein 12
  • ANAC012
  • NAC012
  • NST3
  • SND1
  • NAC SECONDARY WALL THICKENING PROMOTING 3
  • SECONDARY WALL-ASSOCIATED NAC DOMAIN 1
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G32870
  • NAC domain protein 13
  • ANAC013
  • ANAC13
  • NAC13
  • Arabidopsis NAC domain containing protein 13
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G33060
  • NAC 014
  • ANAC014
  • NAC014
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G34190
  • NAC domain containing protein 17
  • anac017
  • NAC017
  • RAO2
  • regulators of aox1a 2
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G52880
  • ATNAM
  • ANAC018
  • NAM
  • NARS2
  • Arabidopsis NAC domain containing protein 18
  • NO APICAL MERISTEM
  • NAC-REGULATED SEED MORPHOLOGY 2
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G52890
  • ANAC19
  • NAC domain containing protein 19
  • ANAC019
  • NAC019
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G54330
  • NAC domain containing protein 20
  • ANAC020
  • NAC020
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G61110
  • NAC domain containing protein 25
  • NAC25
  • anac025
  • NAC025
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G69490
  • NAC-LIKE, ACTIVATED BY AP3/PI
  • ANAC029
  • ATNAP
  • NAP
  • Arabidopsis NAC domain containing protein 29
    		•  transgene SIGnAL TAIR ARAPORT
    AT1G76420
  • NAC368
  • ANAC031
  • CUC3
  • Arabidopsis NAC domain containing protein 31
  • CUP SHAPED COTYLEDON3
    		•  transgene SIGnAL TAIR ARAPORT
    AT2G18060
  • ANAC037
  • VND1
  • Arabidopsis NAC domain containing protein 37
  • vascular related NAC-domain protein 1
    		•  transgene SIGnAL TAIR ARAPORT
    AT2G24430
  • NAC domain containing protein 38
  • ANAC038
  • ANAC039
  • NAC038
  • Arabidopsis NAC domain containing protein 39
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G04060
  • NAC domain containing protein 46
  • anac046
  • NAC046
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G04070
  • NAC domain containing protein 47
  • SPEEDY HYPONASTIC GROWTH
  • ANAC047
  • NAC047
  • SHG
  • SHYG
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G10480
  • NAC50
  • NAC domain containing protein 50
  • ANAC050
  • NAC050
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G10490
  • NAC domain containing protein 52
  • ANAC051
  • ANAC052
  • NAC052
  • SGS1
  • Arabidopsis NAC domain containing protein 51
  • SUPPRESSOR OF GENE SILENCING 1
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G10500
  • NAC domain containing protein 53
  • AIF
  • ANAC053
  • NAC053
  • NTL4
  • ANTHER INDEHISCENCE FACTOR
  • NAC with transmembrane motif 1-like 4
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G12977
  • NAC1L
  • NAC1 like
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G15170
  • ATNAC1
  • ANAC054
  • CUC1
  • Arabidopsis NAC domain containing protein 54
  • CUP-SHAPED COTYLEDON1
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G15500
  • ANAC55
  • NAC domain containing protein 3
  • NAC domain containing protein 55
  • ANAC055
  • ATNAC3
  • NAC055
  • NAC3
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G15510
  • NAC domain containing protein 2
  • ANAC056
  • ATNAC2
  • NAC2
  • NARS1
  • Arabidopsis NAC domain containing protein 56
  • NAC-REGULATED SEED MORPHOLOGY 1
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G17730
  • NAC domain containing protein 57
  • anac057
  • NAC057
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G29035
  • NAC domain containing protein 3
  • ANAC059
  • ATNAC3
  • NAC3
  • ORS1
  • Arabidopsis NAC domain containing protein 59
  • ORE1 SISTER1
    		•  transgene SIGnAL TAIR ARAPORT
    AT3G49530
  • NAC domain containing protein 62
  • ANAC062
  • NAC062
  • NTL6
  • NTM1 (NAC WITH TRANSMEMBRANE MOTIF 1)-LIKE 6
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G01520
  • NAC domain containing protein 67
  • anac067
  • NAC067
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G01540
  • ANAC068
  • NTM1
  • Arabidopsis NAC domain containing protein 68
  • NAC with transmembrane motif1
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G01550
  • NTL13
  • NAC domain containing protein 69
  • ANAC069
  • NAC069
  • NTM2
  • NAC with Transmembrane Motif 2
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G10350
  • NAC domain containing protein 70
  • ANAC070
  • BRN2
  • NAC070
  • BEARSKIN 2
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G27410
  • ANAC72
  • AtRD26
  • ANAC072
  • RD26
  • Arabidopsis NAC domain containing protein 72
  • RESPONSIVE TO DESICCATION 26
    		•  transgene SIGnAL TAIR ARAPORT
    AT4G35580
  • CBNAC
  • NTL9
  • calmodulin-binding NAC protein
  • NAC transcription factor-like 9
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G04410
  • NAC78
  • NTL11
  • anac078
  • NAC2
  • Arabidopsis NAC domain containing protein 78
  • NAC domain containing protein 2
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G07680
  • ATNAC4
  • NAC4
  • NAC domain containing protein 80
  • ANAC079
  • ANAC080
  • NAC080
  • Arabidopsis NAC domain containing protein 79
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G08790
  • ATAF2
  • anac081
  • Arabidopsis NAC domain containing protein 81
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G17260
  • NAC domain containing protein 86
  • NAC86
  • anac086
  • NAC086
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G18270
  • ANAC087
  • Arabidopsis NAC domain containing protein 87
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G24590
  • Arabidopsis NAC domain containing protein 91
  • TCV-interacting protein
  • ANAC91
  • ANAC091
  • TIP
  • NAC091
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G39610
  • Arabidopsis NAC domain containing protein 92
  • ORESARA 1
  • NAC domain containing protein 2
  • NAC domain containing protein 6
  • ANAC092
  • ATNAC2
  • ATNAC6
  • NAC2
  • NAC6
  • ORE1
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G46590
  • NAC domain containing protein 96
  • anac096
  • NAC096
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G53950
  • Arabidopsis NAC domain containing protein 98
  • CUP-SHAPED COTYLEDON 2
  • ANAC098
  • ATCUC2
  • CUC2
    		•  transgene SIGnAL TAIR ARAPORT
    AT5G63790
  • NAC domain containing protein 102
  • ANAC102
  • NAC102
    		•  transgene SIGnAL TAIR ARAPORT

    Germplasm

    2

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.113.112805 23956262
    Transgene Description Promoter Reporter Marker
    pRAB18:GFP RAB18 GFP kanamycin
    pFH0289 RNAi construct BASTA

    Quality Control Comments

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