Name / Stock Number: CS2105626

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NASC stock number: N2105626

other name: UBQ10prom::2xCyPet-2xFYVE(HRS)

donor stock number: P18C

Resource Type: seed

Availability: available

Donors:
  • Yvon Jaillais

Donation Date: 10/27/2014

Date Released: 11/13/2014

Description:

PIPline Marker line. Genetically encoded biosensor for PI3P (phosphatidylinositol 3-phosphate) is fused with 2xCyPET (cyan fluorescent protein), cloned into a multisite Gateway destination vector (pK7m34GW), and expressed under control of the UBQ10 promoter. The ligand-binding domain (LBD) that interacts with PI3P is FYVE; the LBD is from the protein: HRS (Mus musculus). Kanamycin resistant. Homozygous for the transgene.

Growth Requirement: none

Marker: kanamycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Germplasm

Ploidy: 2

2xCyPET localization in late endosome and in the tonoplast in some cells

Taxon: Arabidopsis thaliana 3702

Publications

DOI PubMed
10.1111/tpj.12358 24147788

External links

Transgenes

Transgene Description Promoter Reporter Marker
UBQ10prom::2xCyPet-2xFYVE(HRS) UBQ10 CyPet kanamycin

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