donor stock number: ProSTM-deltaCRB-CK:GUS
NASC stock number: N68960
other name: ProSTM-dCRB-CK:GUS
Resource Type: seed
Availability: not distributed, no seeds at ABRC
Donors:- Neelima Sinha
Donation Date: 02/04/2015
Date Released: 03/04/2015
Description:
Transgenic line; With respect to the start codon, an internal deletion of the core RB-box and core K-box in the STM promoter was generated at position -2004 to -1987 and -268 to -239 bp using overlapping PCR, with ProSTM- K:GUS as template. The Col-0 plant was transformed using the floral dip method. Kanamycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
GUS expression in the abaxial side of growing leaves.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1104/pp.114.248625 | 25524441 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| ProSTM-deltaCRB-CK:GUS | internal deletion of the core RB-box and core K-box in the STM promoter fused to GUS | STM-deltaCRB-CK | GUS | kanamycin |
Quality Control Comments
There is no quality control data for this stock.