donor stock number: hgf1-4
NASC stock number: N69635
Resource Type: seed
Availability: available
Donors:- Marjori Matzke
Donation Date: 11/08/2016
Date Released: 01/31/2017
Description:
Isolated from a population of EMS-mutagenized seedlings homozygous for the T locus (CS69640) in a screen for enhanced expression of an alternatively spliced GFP reporter gene. hgf1-4 is a G775A nucleotide substitution creating a premature termination codon R176STOP. This mutation destroys a splice site. The full DNA sequence of the T locus (Target transgene) can be found under the NCBI accession number, HE582394.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Short delay in flowering, and a Hyper-GFP phenotype (stronger GFP expression than wildtype) in the meristem regions at the shoot and root apices and in the hypocotyl of young seedlings.
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1534/genetics.116.190751 | 27317682 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| Target | GFP | kanamycin |
Quality Control Comments
There is no quality control data for this stock.