ABRC
donor stock number: pTIR1:TIR1-Myc#2-1 AXR3NT-GUS
NASC stock number: N69668
other name: MP2112
Resource Type: seed
Availability: available
Donors:- Mike Prigge
- Mark Estelle
Donation Date: 11/09/2016
Date Released: 02/22/2017
Description:
Transgenic line; The pTIR1:TIR1-Myc construct was made by introducing a 2-kb 5' upstream region of the TIR1 gene adjacent to the TIR1 cDNA into pGWB16 vector and transformed into the AXR3NT-GUS (Col-0) background (CS69686). Hygromycin resistant. Homozygous for the transgene.
Growth Requirement: none
Marker: hygromycin
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1104/pp.113.215582 | 23539280 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| HS::AXR3NT-GUS | 400 base pair fragment of soybean heat shock promoter HS6871, to domains I and II of AXR3 and GUS reporter | 400 base pair fragment of soybean heat shock promoter HS6871 fused to domains I and II of AXR3 | GUS | kanamycin |
| pTIR1:TIR1-Myc | 2-kb 5 upstream region of the TIR1 gene fused to the TIR1 cDNA with a myc tag | TIR1 | hygromycin |
Quality Control Comments
There is no quality control data for this stock.