donor stock number: DR5rev::GFP
NASC stock number: N9361
Resource Type: seed
Availability: available
Donors:- Jiri Friml
Donation Date: 03/11/2005
Date Released: 03/15/2005
Description:
Transgenic line DR5rev::GFP (ER-targeted); GFP fluorescence marks places of auxin activity; sulfonamide resistant
Growth Requirement: sulfonamide resistant
Marker: sulfonamide
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1016/S0092-8674(03)00924-3 | 14651850 |
| 10.1038/nature02085 | 14614497 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| DR5rev::GFP | CaMV35S minimal promoter fused to nine repeats of the DR5 auxin response element (TGTCTC) in inverse orientation fused to ER localized GFP (Clontech) | CaMV35S | GFP | sulfonamide |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Researcher reported zero germination | ABRC | 10/03/2017 | To view detailed data, click here |
| Germination was found to be 91%, which is within the acceptable range for germination percentage (81-100%) set by ABRC. | ABRC | 10/20/2017 | To view detailed data, click here |