NASC stock number: N581346
Resource Type: seed
Availability: available
Donors:- Joseph Ecker
Donation Date: 02/14/2003
Date Released: 02/21/2003
Description:
Sequence-indexed T-DNA insertion line, segregating for annotated insertion; kanamycin was employed for selection of T1 plants; distribution seeds are T2 or T3 generation.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment: NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating.; Please cite the Alonso et al. reference linked to this stock and acknowledge ABRC for distributing the seeds in all presentations and publications utilizing this material.
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Polymorphisms
| Polymorphism | Mutagen | Genotype | Inheritance | Gene | Evidence attribution |
|---|---|---|---|---|---|
| SALKSEQ_081346.2 | T-DNA insertion | segregating | SIGnAL |
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1126/science.1086391 | 12893945 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute | ABRC | 03/10/2015 | To view detailed data, click here |