Name / Stock Number: SAIL_215_G08 / CS810061

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NASC stock number: N810061

Resource Type: seed

Availability: available

Donors:
  • Syngenta, USA

Donation Date: 03/01/2004

Date Released: 06/01/2004

Description:

T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT5G10180
  • AST68
  • SULTR2;1
  • ARABIDOPSIS SULFATE TRANSPORTER 68
  • sulfate transporter 2;1
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT2G37950 polymorphism SIGnAL TAIR ARAPORT
    AT2G08895 polymorphism SIGnAL TAIR ARAPORT
    AT5G10170
  • ATMIPS3
  • MIPS3
  • MYO-INOSITOL-1-PHOSTPATE SYNTHASE 3
  • myo-inositol-1-phosphate synthase 3
    		•  polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    SAILSEQ_215_G08.2 T-DNA insertion segregating
  • AT5G10170
  • AT5G10180
    		• SIGnAL
    SAIL_215_G08 T-DNA insertion segregating
  • AT2G08895
  • AT2G37950
    		• SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.004630 12468722
    Transgene Description Promoter Reporter Marker
    pCSA110 T-DNA LAT52 GUS BASTA

    Quality Control Comments

    Add your quality control comment here
    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here