NASC stock number: N814516
Resource Type: seed
Availability: available
Donors:- Syngenta, USA
Donation Date: 03/01/2004
Date Released: 06/01/2004
Description:
T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.
Growth Requirement: none
Marker: BASTA
Background: Col (Columbia)
ABRC Comment:
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
2
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1105/tpc.004630 | 12468722 |
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pCSA110 | T-DNA | LAT52 | GUS | BASTA |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute | ABRC | 03/10/2015 | To view detailed data, click here |