ABRC
NASC stock number: N659698
Resource Type: seed
Availability: not distributed, Available by special request. Two or more researchers have reported a problem with this line. Contact ABRC for details.
Donors:- Joseph Ecker
- SALK Confirmed T-DNA Project
Donation Date: 02/06/2007
Date Released: 02/09/2007
Description:
Sequence-indexed T-DNA insertion line; confirmed line isolated from original SALK line; presence of the insertion was analyzed by PCR.
Growth Requirement: none
Marker:
Background: Col (Columbia)
ABRC Comment: NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating.; Please cite the Alonso et al. reference linked to this stock and acknowledge ABRC for distributing the seeds in all presentations and publications utilizing this material.
Format Shipped: 100 seeds per vial
Base / Commercial Price: $15 / $120
Germplasm
Ploidy: 2
Taxon: Arabidopsis thaliana 3702
Publications
| DOI | PubMed |
|---|---|
| 10.1126/science.1086391 | 12893945 |
Transgenes
| Transgene | Description | Promoter | Reporter | Marker |
|---|---|---|---|---|
| pROK2 | T-DNA | kanamycin |
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Rob Last genotyping results: homozygous and wildtype | ABRC | 06/12/2014 | To view detailed data, click here |
| Researcher reported low germination | ABRC | 04/01/2015 | To view detailed data, click here |
| Previous low germination stock was replaced with a stock that the germination was found to be 94%. | ABRC | 02/28/2018 | To view detailed data, click here |
| 2 or more researchers reported a problem finding the insert in SALK_017756.37.10.X. | ABRC | 06/05/2024 | To view detailed data, click here |
| Genotypes as homozygous wild type. JA levels and responses are wild type. | user | 06/04/2024 | To view detailed data, click here |