Name / Stock Number: SAIL_529_D11 / CS822416

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NASC stock number: N822416

Resource Type: seed

Availability: available

Donors:
  • Syngenta, USA

Donation Date: 03/01/2004

Date Released: 06/01/2004

Description:

T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT2G45330
  • emb1067
  • TRPT
  • embryo defective 1067
  • 2' tRNA phosphotransferase
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT1G05785 polymorphism SIGnAL TAIR ARAPORT
    AT4G22120
  • CSC1
  • OSCA1.2
  • Calcium permeable Stress-gated cation Channel 1
  • REDUCED HYPEROSMOLALITY-INDUCED Ca2+ INCREASE 1.2
    		•  polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    SAILSEQ_529_D11.1 T-DNA insertion segregating
  • AT1G05785
    		• SIGnAL
    SAILSEQ_529_D11.2 T-DNA insertion segregating
  • AT4G22120
    		• SIGnAL
    SAIL_529_D11 T-DNA insertion segregating
  • AT2G45330
    		• SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.004630 12468722
    Transgene Description Promoter Reporter Marker
    pDAP101 T-DNA BASTA

    Children:

    CS927213

    Quality Control Comments

    Add your quality control comment here
    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here