Resource Type: plasmid
Availability: available
Donors:- Tim Gookin
- Sarah Assmann
Donation Date: 11/06/2014
Date Released: 11/14/2014
Description:
pDOE-06 is a single-vector self-contained BiFC construct based on the mVenus210 fragment system, and the specific fusion orientation for this construct is X::NmVenus210--CVenus210::X. The linker for the X::CVenus210 fragment contains a StrepII-FLAG-StrepII linker for detection or pull-downs. The dual ORF expresssion cassettes are driven by identical single 35S promoters with fused Omega viral translation enhancers. The pDOE-06 construct also features an independent integrated cassette that expresses the XT-Golgi-mTq2 marker from the MAS 2' promoter to specifically label the medial Golgi cisternae with mTurquoise2. Bacterial selection: Kanamycin (50 ug/mL).
Growth Requirement: grow in LB media at 37C overnight
Marker: kanamycin
Background:
ABRC Comment: For academic or non-profit research purposes only. CAMBIA agreement will be sent by email when stock is ordered. Vector nomenclature: X is used in a vector name to signify an empty MCS and to emphasize that the MCS and encoded linkers are translated along with the specified tag. For example, pDOE01-X::NmVen-X::CVen details the vector number (01), the translated product of MCS1 (MCS1 and linkers fused to the NmVenus fragment), and the translated product of MCS3 (MCS3 and linkers fused to the NmVenus fragment).
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter: CaMV 35S fused to Omega 5 prime translation enhancer
Reporter: mVenus210, mTurquoise2
Type: cloning vector
E. coli Top-10
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/tpj.12639 | 25187041 |
Sequence at Genbank: KM507047
Quality Control Comments
| Comment | Commented by | Date | |
|---|---|---|---|
| Several researchers have reported unexpected digest patterns in pDOE vectors with some enzymes (EcoRI and NcoI). ABRC QC could not find any problems. Donor (Tim Gookin) has confirmed stocks are functional regardless and is aware of these issues. | ABRC | 12/30/2015 | To view detailed data, click here |
Note
CAMBIA2NOTICE Cambia vectors: The gus gene in this material is protected under US patent Nos. 5,268 and 5,599,670 and GB patent no. 2,197,653C assigned to Cambia Biosystems LLC. Other components of these vectors may be protected by rights held by other parties.
USAGE RESTRICTIONS:These vectors are provided for academic or non-profit research purposes only. By accepting or using these vectors you agree to be bound by the conditions of this notice. You may refuse to accept the conditions of this notice by returning this material to CAMBIA unused.
Academic and Non-Profit Laboratories:No CAMBIA vectors can be distributed further to third parties outside your laboratory unless the recipient receives a copy of this notice and agrees to be bound by its terms.
Commercial Laboratories: A license is required for use of the gus gene within the CAMBIA vectors for research or production purposes by any commercial entity. Information about licenses may be obtained from Cambia Biosystems LLC, PO Box 97022 Bellevue, WA 98009 U. S. A. Tel: 1 206 453 1600, Fax: 1 206 455 4105, Email: raj@cambia.org.au. Other components or uses of these vectors may require additional licenses from other patent holders.
GENERAL: Users are responsible for ensuring that all the relevant licenses are obtained from the patent holders for any commercial or for-profit research use of these vectors. Users will cite the provisions of these vectors in their published works. Use of these vectors for cloning purposes shall explicitly state that the vector was constructed in pCAMBIA.... as a part of the description of the new cloning. No vector derived from such cloning should retain only a pCAMBIA designation, as these designations are reserved for vectors developed and distributed by CAMBIA and its partners. Users may refer to the forthcoming publication by Roberts C S, Rajagopal S, Yang W, Nugroho S, Smith L, Nguyen T, Ravi K S, Kilian A and Jefferson R A*. Until publication, reference may be made to the informal presentation "A comprehensive new set of modular vectors to allow both routine and advanced manipulations and efficient transformation of rice by both Agrobacterium and direct gene transfer methods" made at the Rokefeller Foundation Meeting of the International Program on Rice Biotechnology, 15-19 September 1997, Malacca, Malaysia. *Corresponding author. CAMBIA, GPO Box 3200, Canberra City, ACT 2601, Australia.
WARRANTY: This material is provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied, and without any representation or warranty that the use or supply of this material will not infringe on any patent, copyright, trademark, or other right. CAMBIA and its employees and agents shall not be held liable for any use of the material transferred to you under this notice. You agree that to the extent permitted by law you will indemnify or hold harmless CAMBIA and its employees and agents for any loss, claim, damage or liability, of whatsoever kind or nature, which may arise from acceptance, use, handling or storage of the material by you.