Name / Stock Number: pFGC4633 / CD3-493

Resource Type: plasmid

Availability: available

Donors:
  • Natalie Doetsch
  • Richard Jorgensen
  • Chromatin Functional Genomics Consortium

Donation Date: 06/18/2003

Date Released: 07/17/2003

Description:

RNAi construct contains mas3' UTR, HPT, mas1' promoter, CaMV 35S promoter, omega leader, partial NFC04 (sense), CHSA intron, partial NFC04 (antisense), ocs3' UTR in pFGC1008 vector

Growth Requirement: grow in LB media at 37 C overnight

Marker: chloramphenicol

Background:

ABRC Comment: For academic or non-profit research purposes only. CAMBIA agreement will be sent by email when stock is ordered.

Format Shipped: bacterial stab

Base / Commercial Price: $15 / $120

Plasmid

Promoter:

Reporter:

Type: RNAi clone

E. coli DH5-alpha

Arabidopsis thaliana 3702

Additional Information

Quality Control Comments

There is no quality control data for this stock.

Note

CAMBIA

NOTICE: University of Arizona derivatives of CAMBIA vectors (Please see below the notice from CAMBIA regarding their vectors, which also applies to use of derivatives)

Components of these vectors may be protected by rights held by other parties. Components or uses of these vectors may require licenses from patent holders. These vectors are provided for academic or non-profit research purposes only. By accepting or using these vectors you agree to be bound by the conditions of this notice. You may refuse to accept the conditions of this notice by returning this material unused. No CAMBIA vector derivatives may be distributed further to third parties outside your laboratory unless the recipient receives a copy of this notice and the accompanying CAMBIA notice and agrees to be bound by their terms. Users are responsible for ensuring that all relevant licenses are obtained from the patent holders for any commercial or for profit research use of these vectors. Users will cite the provision of these vectors in their published works. The appropriate citation for these vectors will be posted at our web site once they are published them. Prior to our publication of these vectors please cite Carolyn Napoli, Ross Atkinson and our web site, ChromDB: http://www.chromdb,org.

WARRANTY:This material is provided without warranty for merchantability or fitness for a particular purpose or any other warranty, express or implied, and without any representation or warranty that the use or supply of the material will not infringe any patent, copyright, trademark, or other right. CAMBIA, the University of Arizona, and their employees and agents shall not be held liable for any use of material transferred to you under this notice. You agree that to the extent permitted by law you will indemnify and hold harmless CAMBIA, the University of Arizona, and their employees and agents for any loss, claim, damage or liability, of whatsoever kind or nature, which may arise from acceptance, use, handling or storage of the material by you.

NOTICE Cambia vectors: The gus gene in this material is protected under US patent Nos. 5,268 and 5,599,670 and GB patent no. 2,197,653C assigned to Cambia Biosystems LLC. Other components of these vectors may be protected by rights held by other parties.

USAGE RESTRICTIONS: These vectors are provided for academic or non-profit research purposes only. By accepting or using these vectors you agree to be bound by the conditions of this notice. You may refuse to accept the conditions of this notice by returning this material to CAMBIA unused.

Academic and Non-Profit Laboratories: No CAMBIA vectors can be distributed further to third parties outside your laboratory unless the recipient receives a copy of this notice and agrees to be bound by its terms.

Commercial Laboratories: A license is required for use of the gus gene within the CAMBIA vectors for research or production purposes by any commercial entity. Information about licenses may be obtained from Cambia Biosystems LLC, PO Box 97022 Bellevue, WA 98009 U. S. A. Tel: 1 206 453 1600, Fax: 1 206 455 4105, Email: raj@cambia.org.au. Other components or uses of these vectors may require additional licenses from other patent holders.

GENERAL: Users are responsible for ensuring that all the relevant licenses are obtained from the patent holders for any commercial or for-profit research use of these vectors. Users will cite the provisions of these vectors in their published works. Use of these vectors for cloning purposes shall explicitly state that the vector was constructed in pCAMBIA.... as a part of the description of the new cloning. No vector derived from such cloning should retain only a pCAMBIA designation, as these designations are reserved for vectors developed and distributed by CAMBIA and its partners. Users may refer to the forthcoming publication by Roberts C S, Rajagopal S, Yang W, Nugroho S, Smith L, Nguyen T, Ravi K S, Kilian A and Jefferson R A*. Until publication, reference may be made to the informal presentation 'A comprehensive new set of modular vectors to allow both routine and advanced manipulations and efficient transformation of rice by both Agrobacterium and direct gene transfer methods' made at the Rockefeller Foundation Meeting of the International Program on Rice Biotechnology, 15-19 September 1997, Malacca, Malaysia. *Corresponding author. CAMBIA, GPO Box 3200, Canberra City, ACT 2601, Australia.

WARRANTY: This material is provided without warranty of merchantability or fitness for a particular purpose or any other warranty, express or implied, and without any representation or warranty that the use or supply of this material will not infringe on any patent, copyright, trademark, or other right. CAMBIA and its employees and agents shall not be held liable for any use of the material transferred to you under this notice. You agree that to the extent permitted by law you will indemnify or hold harmless CAMBIA and its employees and agents for any loss, claim, damage or liability, of whatsoever kind or nature, which may arise from acceptance, use, handling or storage of the material by you.