NASC stock number: N781549
donor stock number: tag only
Resource Type: plasmid
Availability: not distributed
Donors:- Niko Geldner
Donation Date: 11/01/2012
Date Released: 11/06/2012
Description:
Wave DNA line, a subcellular marker line with spectrally distinct, fluorescent fusion construct providing cytosolic and nuclear localization. The construct is under the control of thepUBQ10 promoter. Based on the pNIGEL recombination vector pNIGEL 17 a fusion construct was generated using an ORF clone from the SSP consortium. The marker constructs are available in four colors Blue (mCerulean), Bluegreen (mTFP1), Yellow (YFP) and Red (mCherry). This construct utilizes the red fluorescent marker (mCherry).
Growth Requirement: grow in LB media at 37C overnight
Marker: kanamycin
Background: Col (Columbia)
ABRC Comment: Please note that this WAVE clone is based on pGreen II, which is unable to replicate in Agrobacterium without the presence of another binary plasmid, pSoup (order as stock number CD3-1124).
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter:
Reporter: mCherry
Type: ORF expression clone
Arabidopsis thaliana 3702
Additional Information
| DOI | PubMed |
|---|---|
| 10.1111/j.1365-313X.2009.03851.x | 19309456 |
Quality Control Comments
There is no quality control data for this stock.
