Name / Stock Number: SAIL_840_B04 / CS837516

NASC stock number: N837516

Resource Type: seed

Availability: available

Donors:
  • Syngenta, USA

Donation Date: 03/01/2004

Date Released: 06/04/2004

Description:

T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: BASTA

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT2G26780 polymorphism SIGnAL TAIR ARAPORT
AT1G60995
  • HISE1
  • high sterol ester 1
  • polymorphism SIGnAL TAIR ARAPORT
    AT2G08180 polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    SAILSEQ_840_B04.1 T-DNA insertion segregating
  • AT1G60995
  • SIGnAL
    SAIL_840_B04 T-DNA insertion segregating
  • AT2G08180
  • AT2G26780
  • SIGnAL

    Arabidopsis thaliana 3702

    Additional Information

    DOI PubMed
    10.1105/tpc.004630 12468722
    Transgene Description Promoter Reporter Marker
    pDAP101 T-DNA BASTA

    Quality Control Comments

    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here