Name / Stock Number: WiscDsLox302H10 / CS850925

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NASC stock number: N850925

Resource Type: seed

Availability: available

Donors:
  • Patrick Krysan
  • Michael Sussman
  • Rick Amasino

Donation Date: 09/21/2004

Date Released: 12/01/2004

Description:

Sequence-indexed T-DNA insertion line; the T-DNA includes a Ds transposon and a LoxP recombination site. A Basta resistance marker is used to select for the T-DNA and for the Ds transposon following any transposition. However, be aware that lines from this set might not be able to launch their Ds element. Be sure to test any line for the ability to transpose before using it in experiments. Hygromycin resistance can be used for selection of lines where the Ds element has been excised by transposition. WiscDsLox lines do not contain Ac transposase, and the lines should be crossed to a line expressing Ac transposase in order to mobilize the Ds transposon. May be segregating for phenotypes that are not linked to the insertion. May have additional insertions potentially segregating.

Growth Requirement: none

Marker: hygromycin

Background: Col (Columbia)

ABRC Comment:

Format Shipped: 100 seeds per vial

Base / Commercial Price: $15 / $120

Genes

Gene Names Evidence
AT5G25540
  • CTC-interacting domain 6
  • CID6
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT3TE47130 polymorphism SIGnAL TAIR ARAPORT
    AT3TE59195 polymorphism SIGnAL TAIR ARAPORT
    AT4TE33030 polymorphism SIGnAL TAIR ARAPORT
    AT3G11510
  • uS11y
  • Ribosomal Protein uS11y
    		•  polymorphism SIGnAL TAIR ARAPORT
    AT3G11505 polymorphism SIGnAL TAIR ARAPORT

    Germplasm

    2

    Polymorphisms

    Polymorphism Mutagen Genotype Inheritance Gene Evidence attribution
    WISCDSLOX302H10 T-DNA insertion segregating
  • AT5G25540
    		• SIGnAL
    Wiscseq_DsLox302H10.4 T-DNA insertion segregating
  • AT3G11505
  • AT3G11510
    		• SIGnAL
    Wiscseq_DsLox302H10.1 T-DNA insertion segregating
  • AT3TE47130
    		• SIGnAL
    Wiscseq_DsLox302H10.3 T-DNA insertion segregating
  • AT3TE59195
    		• SIGnAL
    Wiscseq_DsLox302H10.5 T-DNA insertion segregating
  • AT4TE33030
    		• SIGnAL
    WISCSEQ_DSLOX302H10.6 T-DNA insertion segregating
    WISCSEQ_DSLOX302H10.2 T-DNA insertion segregating

    Arabidopsis thaliana 3702

    Additional Information

    Transgene Description Promoter Reporter Marker
    pDs-Lox T-DNA includes a Ds transposon and a LoxP recombination site CaMV35S, MAS LUC BASTA, hygromycin

    Quality Control Comments

    Add your quality control comment here
    Comment Commented by Date
    new T-DNA insertions have been identified in this stock by TDNA-Seq, a method for next-generation sequencing performed in J. Ecker lab at SALK Institute ABRC 03/10/2015 To view detailed data, click here