Name / Stock Number: Universal_AraYpet-FRT-tetA-FRT / CD3-2819

donor stock number: JMA619

Resource Type: plasmid

Availability: available

Donors:
  • Jose Alonso

Donation Date: 08/28/2019

Date Released: 10/18/2019

Description:

AraYpet-FRT-TetA-FRT recombineering cassette. To generate this cassette, the tetracycline resistance gene, TetR, and corresponding promoter sequences were amplified from the genomic DNA of E. coli recombineering strain SW102, and the PCR product was then employed in a recombineering reaction to insert the FRT-Tet-FRT sequences in the Universal AraYpet cassette.

Growth Requirement: grow in LB media at 32C for 48 hours

Marker: kanamycin

Background:

ABRC Comment:

Format Shipped: bacterial stab

Base / Commercial Price: $15 / $120

Plasmid

Promoter:

Reporter:

Type: cloning vector

E. coli SW105

Additional Information

Quality Control Comments

There is no quality control data for this stock.