donor stock number: JMA1808
Resource Type: plasmid
Availability: available
Donors:- Jose Alonso
Donation Date: 08/28/2019
Date Released: 10/18/2019
Description:
Spectinomycin-Kanamycin recombineering cassette. The aadA sequences were amplified from the pTF101 vector with the primers SpectFKan and SpectRKan and inserted in the JAtY63D14 clone to generate the cassette template. Primers Spect-Kan test F and Spect-Kan test R can be used to amplify the cassette from the cassette template.
Growth Requirement: grow in LB media at 32C for 48 hours
Marker: kanamycin
Background:
ABRC Comment:
Format Shipped: bacterial stab
Base / Commercial Price: $15 / $120
Plasmid
Promoter:
Reporter:
Type: cloning vector
E. coli SW105
Additional Information
Quality Control Comments
There is no quality control data for this stock.